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KMID : 1003220000060010215
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Journal of Korean Oriental Chronic Disease
2000 Volume.6 No. 1 p.215 ~ p.222
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The Role of Water Extract of Ganoderma lucidum-Induced Angiogenesis
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안교필/Ahn, Kyo Pil
김동원/김형환/최호영/Kim, Dong Won/Kim, Hyung Hwan/Choi, Ho Young
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Abstract
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Objectives : To discover and test the efficacy of new angiogenic materials, The angiogenic effect of the water extract of Ganoderma lucidum (EGL) was measured under rat sponge model for quantitative in-vivo studies.
Methods : It was based on subcutaneous implantation of sterile polyester sponges in the male sprague dawley (SD) rats and we have characterized a dye method and histology. The angiogenic response was verified by quantitation of histological evaluation of vascularized sponges. Angiogenesis was assessed by the dye method that incorporate 5% carmine red (490 nm), 1% cresyl violet (609 nm) in 10% gelatin solution. It was used to monitor at fixed time points (days 7 and 14). vascularity index expressed as ㎍ dye/㎎ dry tissue.
Results : Subcutaneous implantation of sterile polyester sponges in SD rats enhanced the neovascularization between days 7 and 14. Daily doses of 1 μ㎎/㎏ and 10 μ㎎/㎏ EGL produced no apparent effect on spongeinduced angiogenesis but 100, 300 and 1000 ㎍/㎏ EGL caused intense neovasculalization. The vasoconstrictor peptide, angiotensin Ⅱ, in daily doses of 100 nmol, caused an intense neovascularization but lower doses of angiotensin Ⅱ produced no apparent effect (1 nmol, 10 nmol). 100 ㎍/㎏ EGL - induced angiogenesis represents a special form of angiogenesis and We have shown that co-administration of 100 ㎍/㎏ EGL and 100 nmol All accelerated potent angiogenesis.
Conclusion : Thus we concluded that the EGL was possessed intense angiogenesis and EGL may have therapeutic potential for the management of endometrial development, wound repair and embryonic development.
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KEYWORD
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